Skip to main content
. Author manuscript; available in PMC: 2024 Nov 1.
Published in final edited form as: J Immunol. 2023 Nov 1;211(9):1340–1347. doi: 10.4049/jimmunol.2300317

Figure 3. Jagged1 deficiency enhances TNF-mediated osteoclastogenesis.

Figure 3

A) qPCR analysis of Jag1 expression in BMMs of the WT control (Ctrl) and Jag1ΔM/ΔM mice, B) Osteoclast differentiation using BMMs (3.125 × 104/cm2) derived from WT control (Ctrl) and Jag1ΔM/ΔM mice was stimulated with and without TNFα for 5 days. Left: TRAP staining. Scale bar: 200 μm. Right: TRAP-positive multinucleated cells (MNCs) (≥3 nuclei/cell) per well (n=4/group). C) Von Kossa staining of osteoclast differentiation cultures induced by TNF for 14 days. Mineralized area: black; resorption area: white. D) Immunoblot analysis of Blimp1, Nfatc1, c-Fos expression in the Ctrl and Jag1ΔM/ΔM BMMs with TNFα treatments for the indicated time periods. p38 was blotted as the loading control. The relative density of each cFos band to its corresponding loading control p38 band was calculated by Image J software, and then was normalized to the WT controls at time 0 (the 1st lane). E) Schematic of TNF-induced feedback inhibition of osteoclast differentiation of macrophages.