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. Author manuscript; available in PMC: 2024 Nov 1.
Published in final edited form as: J Immunol. 2023 Nov 1;211(9):1340–1347. doi: 10.4049/jimmunol.2300317

Figure 4. Overexpression of Jagged1 suppresses inflammatory osteoclastogenesis.

Figure 4

A) Osteoclast differentiation using BMMs (6.25 × 104/cm2) derived from WT control (Ctrl) and Jag1mTg mice were stimulated with TNFα for 4 days with or without TGFβ priming for 3 days. Left: TRAP staining. Scale bar: 200 μm. Right: TRAP-positive MNCs (≥3 nuclei/cell) per well (n=4/group). B) Immunoblot analysis of Jagged1 and Blimp1 (top), and Nfatc1 (bottom) expression in Ctrl and Jag1mTg BMMs with TNFα treatments for the indicated time periods, with or without TGFβ priming. p38 was blotted as the loading control.