Table 3.
Decreased proteins in macrophages infected with live Paracoccidioides brasiliensis yeast cells involved in stress response.
| Accession number1 | Protein description and biological process2 | Score3 | Fold change4 |
|---|---|---|---|
| Stress response | |||
| A0A7I2V2S7 | 75 kDa glucose-regulated protein | 7343.21 | 0.677 |
| P04040 | Catalase | 6229.48 | * |
| P00390 | Glutathione reductase_ mitochondrial | 3504.57 | * |
| P78417 | Glutathione S-transferase omega-1 | 1469.15 | * |
| Q5TA02 | Glutathione-dependent dehydroascorbate reductase (Fragment) | 1469.15 | * |
| A8MX94 | GST class-pi | 21612.53 | 0.386 |
| A0A6Q8PGK1 | Heat shock 27 kDa protein | 30162.08 | * |
| H0Y8K0 | Heat shock 70kDa protein 9B (Mortalin-2)_ isoform CRA_a | 5920.49 | * |
| P04792 | Heat shock protein beta-1 | 30168.58 | * |
| H3BSU0 | Liver carboxylesterase 1 (Fragment) | 407.80 | * |
| P55145 | Mesencephalic astrocyte-derived neurotrophic factor | 12025.95 | * |
| G5E977 | Nicotinate phosphoribosyltransferase | 2537.61 | * |
| A1KZ92 | Peroxidasin-like protein | 344.36 | * |
| A0A0A0MRQ5 | Peroxiredoxin-1 | 6482.96 | * |
| Q13162 | Peroxiredoxin-4 | 8387.69 | * |
| P30044 | Peroxiredoxin-5_ mitochondrial | 1369.47 | * |
| P30041 | Peroxiredoxin-6 | 13986.24 | * |
| P10768 | S-formylglutathione hydrolase | 1667.33 | * |
| P38646 | Stress-70 protein_ mitochondrial | 7419.77 | 0.670 |
| P31948 | Stress-induced-phosphoprotein 1 | 10056.83 | * |
| P00441 | Superoxide dismutase [Cu-Zn] | 8491.53 | * |
| P10599 | Thioredoxin | 7245.89 | * |
| O95881 | Thioredoxin domain-containing protein 12 | 1161.27 | * |
| Q16881 | Thioredoxin reductase 1_ cytoplasmic | 4239.45 | * |
| P30048 | Thioredoxin-dependent peroxide reductase_ mitochondrial | 5641.69 | * |
| A0A182DWI3 | Thioredoxin-disulfide reductase | 4235.96 | * |
1 Accession number of matched protein from Homo sapiens’ macrophages Uniprot database (https://www.uniprot.org/).
2 Proteins annotation from Homo sapiens’ database or by homology in NCBI database (http://www.ncbi.nlm.nih.gov/) and biological process according to the classification of KEGG (https://www.genome.jp/kegg/), UniProt database (https://www.uniprot.org/), NCBI database (http://www.ncbi.nlm.nih.gov/) and CORUM database (http://mips.helmholtz-muenchen.de/corum/).
3 PLGS score is the result of different mathematical models for peptide and fragment assign prediction.
4 Fold-change values were obtained by dividing the values of protein abundance (in fmol) from macrophages during infection by live PB by the abundance in the uninfected macrophages. Proteins with a minimum fold-change of 50% (≤ 0.67) were considered to be dowregulated.
* Proteins detected only in uninfected macrophages.
A fold equal to or less than 0.67 was considered.