Table 5.
Some proteins decreased in macrophages infected vwith Paracoccidioides brasiliensis yeast cells involved in immune response.
| Accession number1 | Protein description and biological process2 | Score3 | Fold change4 |
|---|---|---|---|
| Immune response | |||
| O15143 | Actin-related protein 2/3 complex subunit 1B | 8740.81 | 0.631 |
| O15144 | Actin-related protein 2/3 complex subunit 2 | 3617.00 | * |
| O15145 | Actin-related protein 2/3 complex subunit 3 | 17431.37 | * |
| R4GN08 | Actin-related protein 2/3 complex subunit 4 (Fragment) | 1106.88 | * |
| O15511 | Actin-related protein 2/3 complex subunit 5 | 3627.45 | * |
| F8WE84 | Actin-related protein 3 | 9712.19 | * |
| D6RBL5 | Annexin | 13850.32 | 0.650 |
| P04083 | Annexin A1 | 18072.62 | * |
| P08758 | Annexin A5 | 30602.12 | 0.644 |
| O43150 | Arf-GAP with SH3 domain_ ANK repeat and PH domain-containing protein 2 | 594.14 | * |
| G5E9J0 | Arp2/3 complex 34 kDa subunit | 475.38 | * |
| P61769 | Beta-2-microglobulin | 3926.66 | * |
| A0A7I2V440 | Cathepsin B | 6042.11 | * |
| P25774 | Cathepsin S | 1155.57 | * |
| P36222 | Chitinase-3-like protein 1 | 1597.86 | * |
| Q07021 | Complement component 1 Q subcomponent-binding protein_ mitochondrial | 7230.48 | * |
| A0A7P0TB36 | Endoplasmic reticulum chaperone BiP | 21012.03 | 0.582 |
| P54652 | Heat shock-related 70 kDa protein 2 | 11903.81 | 0.001 |
| P09429 | High mobility group protein B1 | 1668.83 | * |
| C9J0D1 | Histone H2A | 7828.83 | * |
| P0C0S8 | Histone H2A type 1 | 8902.71 | * |
| Q96QV6 | Histone H2A type 1-A | 3271.32 | * |
| P04908 | Histone H2A type 1-B/E | 8902.71 | * |
| Q93077 | Histone H2A type 1-C | 8902.71 | * |
| P20671 | Histone H2A type 1-D | 8902.71 | * |
| Q96KK5 | Histone H2A type 1-H | 8902.71 | * |
| Q99878 | Histone H2A type 1-J | 8902.71 | * |
| Q6FI13 | Histone H2A type 2-A | 8902.71 | * |
| Q8IUE6 | Histone H2A type 2-B | 2747.75 | * |
| Q16777 | Histone H2A type 2-C | 8902.71 | * |
| Q9BTM1 | Histone H2A.J | 8902.71 | * |
| Q71UI9 | Histone H2A.V | 8676.91 | * |
| P0C0S5 | Histone H2A.Z | 8676.91 | * |
| P16104 | Histone H2AX | 3273.08 | * |
| U3KQK0 | Histone H2B | 16807.41 | * |
| Q96A08 | Histone H2B type 1-A | 7130.46 | * |
| P33778 | Histone H2B type 1-B | 17593.68 | * |
| P62807 | Histone H2B type 1-C/E/F/G/I | 16807.41 | * |
| P58876 | Histone H2B type 1-D | 16807.41 | * |
| Q93079 | Histone H2B type 1-H | 16807.41 | * |
| P06899 | Histone H2B type 1-J | 17593.68 | * |
| O60814 | Histone H2B type 1-K | 16807.41 | * |
| Q99880 | Histone H2B type 1-L | 16807.41 | * |
| Q99879 | Histone H2B type 1-M | 16807.41 | * |
| Q99877 | Histone H2B type 1-N | 16807.41 | * |
| P23527 | Histone H2B type 1-O | 17593.68 | * |
| Q16778 | Histone H2B type 2-E | 17593.68 | * |
| A0A2R8Y619 | Histone H2B type 2-E1 | 311.58 | * |
| Q5QNW6 | Histone H2B type 2-F | 16807.41 | * |
| Q8N257 | Histone H2B type 3-B | 17593.68 | * |
| P57053 | Histone H2B type F-S | 16800.03 | * |
| Q6NXT2 | Histone H3.3C | 3853.37 | * |
| P62805 | Histone H4 | 7844.59 | * |
| P05107 | Integrin beta-2 | 2518.87 | * |
| P33241 | Lymphocyte-specific protein 1 | 820.58 | * |
| P14174 | Macrophage migration inhibitory factor | 4042.46 | * |
| Q96E17 | Ras-related protein Rab-3C | 1115.57 | * |
| P19793 | Retinoic acid receptor RXR-alpha | 654.98 | * |
| P28702 | Retinoic acid receptor RXR-beta | 1177.33 | * |
| P48443 | Retinoic acid receptor RXR-gamma | 630.23 | * |
| H0Y858 | Toll-like receptor 9 (Fragment) | 624.89 | * |
| A0A499FJK2 | Transforming growth factor beta | 618.77 | * |
1 Accession number of matched protein from Homo sapiens’ macrophages Uniprot database (https://www.uniprot.org/).
2 Proteins annotation from Homo sapiens’ database or by homology in NCBI database (http://www.ncbi.nlm.nih.gov/) and biological process according to the classification of KEGG (https://www.genome.jp/kegg/), UniProt database (https://www.uniprot.org/), NCBI database (http://www.ncbi.nlm.nih.gov/) and CORUM database (http://mips.helmholtz-muenchen.de/corum/).
3 PLGS score is the result of different mathematical models for peptide and fragment assign prediction.
4 Fold-change values were obtained by dividing the values of protein abundance (in fmol) from macrophages during infection by live PB by the abundance in the uninfected macrophages. Proteins with a minimum fold-change of 50% (≤ 0.67) were considered to be dowregulated.
* Proteins detected only in uninfected macrophages.
A fold equal to or less than 0.67 was considered.