Figure 4.

Verification of the allelic effect of QTL for young fruit resistance identified by QTL-seq analysis. (A) Disease distribution at 5 days post inoculation for the selected F₂ plants (‘Gy 14’ × ‘A4-3’) homozygous for the ‘Gy14’ or ‘DH4-3’ alleles (n=82) (bar graph), and the frequency of the resistant ‘A4-3’ allele on chromosome 5 (black line). Each F₂ value is the mean of 10-30 fruit/plant. (B) Disease scores of F₂ individuals of ‘Gy14’ (pickling cucumber) ‘A4-3’ possessing either the ‘A4-3’ or ‘Gy14’ allele at the predicted QTL at chromosomes 5 (qPFR5.1) and 6. (C) F₃ families of ‘Poinsett 76’ (fresh market cucumber) ‘A4-3’ possessing either the ‘A4-3’ or ‘Poinsett’ allele at qPFR5.1. Each point is the mean of >20 fruits/family from the greenhouse and >50 fruits/family from the field. Ns, non-significant; **P < 0.01; ***P < 0.001.