Figure 8.

QTL-seq analysis for age-related resistance (ARR) to Phytophthora fruit rot using segregating populations derived from ‘Gy14’ (ARR-) × ‘Poinsett 76’ (ARR+). (A) Top: Disease rating distributions for F₂ individuals (n=95). All fruit were harvested at 16-18 dpp. Disease rating on a 1-9 scale. Fruit were scored at 10 dpi. The 15 most resistant and susceptible individuals were selected for QTL-seq. Bottom: QTL-seq analysis. Δ(SNP-index) was calculated with a window size of 2Mb. Horizontal lines represent confidence thresholds of 95% (red) and 99% (blue). (B) Top: Disease rating distributions for doubled haploid lines (n=79, 3-5 fruit/line). Disease ranking on 0-5 scale. Fruit were scored at 7 dpi. The 8 most resistant and susceptible lines were selected for QTL-seq. Bottom: QTL-seq analysis. Δ(SNP-index) was calculated with a window size of 2Mb. Allele frequencies for the resistant and susceptible bulks are provided in Supplementary Figure 1 . (C) Phenotype of ‘Poinsett 76’ fruit at 8 and 16 dpp photographed at 7 days post inoculation (dpi). (D) Mean disease ratings of F4 families (5 plants/F₄ family) homozygous for the ‘Gy14’ or ‘Poinsett 76’ allele within the QTL on chromosome 3. (E) Disease ratings for ‘Poinsett 76’ and ‘A4-3’ fruit harvested at 8 and 16 dpp and scored at 7 dpi. Each value is the mean of 8-14 fruit.