FIG. 2.

SDS-PAGE analysis of the citrate lyase subunits from L. mesenteroides at various steps of purification. Lane 1, molecular weight markers; lane 2, preparation after ammonium sulfate precipitation; lane 3, preparation after elution from hydroxylapatite column; lane 4, preparation after elution from Q-Sepharose column fractions of the citrate lyase. The numbers at the left indicate molecular weights (10³). The band corresponding to the γ subunit, which does not appear in the photograph, was very weakly visible in some preparations.