TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| E. coli | ||
| TG1 | supE hsdΔ5 thi Δ(lac-proAB) F′[traD36 proAB+ lacIqlacZΔM15] | 20 |
| AR1062 | F−thr leu ara azi fhuA lacY tsx minA gal rpsL xyl mtl thi hsdR | 13 |
| BL21(DE3) | hsdS gal (λclts857 ind-1 sam-7 nin-5 lacUV5-T7 gene 1) | 35 |
| L. mesenteroides | ||
| 195 | Chr. Hansen (Milwaukee, Wis.) | |
| 18T | Our collection | |
| Plasmids | ||
| pJDC9 | EmrlacZ | 9 |
| pBluescript KS+ | AprlacZ | Stratagene |
| pCL9 | pJDC9 containing the 4.4-kb Sau3A DNA fragment from L. mesenteroides subsp. cremoris 195 with citDEFG gene cluster | This work |
| pNA2028 | pBluescript carrying the 4.4-kb SacI-XbaI insert of pCL9 under the control of the T7 promoter | This work |
| pNA2030 | pBluescript carrying the citC gene | This work |