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Purification steps of citrate lyase from L. mesenteroides
| Step | Protein (mg) | Total activity (U) | Sp act (U/mg) | Yield (%) | Purifi- cation (fold) |
|---|---|---|---|---|---|
| Crude extract | 377 | 181 | 0.48 | 100 | 1 |
| Protamine sulfate | 182 | 165 | 0.91 | 91.3 | 1.8 |
| Ammonium sulfate | 62 | 130 | 2.10 | 72 | 4.3 |
| Hydroxylapatite | 4.3 | 25 | 5.70 | 13.5 | 11.8 |
| Ion exchange | 1.3 | 16 | 12.10 | 8.8 | 25.3 |
