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. 2023 Dec 1;7(4):046113. doi: 10.1063/5.0163363

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FIG. 1. — Fabricating FRESH™ printed cardiac tissue strips in a 24-well plate format. (a) Timeline of the fabrication process from FRESH™ printing at day 0 to calcium imaging at day 14. (b) Schematic of the cardiac tissue (pink) designed to be FRESH™ printed around support posts (gray). (c) Image of FRESH™ printing using a cell-laden bioink, printing into a single well. (d) Top-down view of cardiac tissues in each well showing the 24-well plate format; inset shows a cardiac tissue in a single well. (e) Schematic of cardiac tissue compaction around support posts over time in culture. (f) Schematic of the cardiac tissue used for high-resolution, high-speed calcium imaging.