TABLE 1.

Definitions of the 11 indicators.

Indicator	Annotation
Raw reads	Original read number of the specimen
10M normalize reads	All reads were homogenized with 10M as the standard
Double discard reads	Reads of potentially contaminating microorganisms in the environment and reagents, and reads of target species brought out by similar sequences of other species were excluded
RPM	Reads per million mapped reads
TPM	Transcript per Kilobase per million mapped reads
Coverage	Proportion of genes sequenced in the entire genome
In Genus Rank	Ranking the species of the same genus detected in the sample, ranked from highest to lowest sequence number
Genus Rank Ratio	The genus in which the microbial belongs is ranked from highest to lowest by the number of sequences in all the genera detected in the sample
King Genus Rank Ratio	Genus ranking ratio in different kingdoms
Genus Rank Ratio*in Genus Rank	Joint indicator
King Genus Rank Ratio*in Genus Rank	Joint indicator

bold values means a significant difference.