Fig. 1.

Knockout of P2rx1 alleviates liver injury in the AILI mouse model. Dynamic hepatic relative P2rx1 mRNA expression and P2RX1 expression in (a) DILI patients and (b) APAP-treated WT mice (n = 4–6 per group); (c) Representative IF staining images of P2RX1 in WT mice with or without AILI (origin magnification × 100, scale bar = 100 μm); (d) Serum ALT and AST levels in WT and P2rx1^−/− mice after a single dose of PBS or APAP (300 mg/kg, n = 4–6 per group); (e) Representative images of H&E staining in liver sections of WT and P2rx1^−/− mice with or without APAP challenge (origin magnification × 100). Quantification of necrotic areas in liver sections by H&E staining (n = 4–6 per group); (f) Survival curve of WT and P2rx1^−/− mice in response to a single lethal dose of APAP (500 mg/kg, n = 12 per group). Data are shown as the means ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001