Fig. 5.

STING activator aggravates liver injury of P2rx1^−/− mice after APAP treatment. (a) A schematic of STING activation by DMX (10 mg/kg) in the APAP overdose model. (b) Serum levels of ALT and AST in P2rx1^−/− mice with or without DMX pretreatment (10 mg/kg, n = 4–6 per group); (c) Representative images of H&E staining (original magnification × 100) and quantification of hepatic necrosis area in P2rx1^−/− mice with or without DMX pretreatment (n = 4–6 per group); (d) Representative images and the quantification of TUNEL-positive cells in liver sections of P2rx1^−/− mice with or without DMX pretreatment (original magnification × 200, n = 4–6 per group); (e) Hepatic MDA and (f) plasma mtDNA levels of P2rx1^−/− mice with or without DMX pretreatment; (g) Representative images of MitoSOX Red probe in primary P2rx1^−/− hepatocytes pretreated with DMSO or DMX. Data are shown as the means ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001