Table 2.
Primer sequences and cycle conditions of RAMS and REP-PCR
| Primer | Sequence | Temperature cycle | References |
|---|---|---|---|
| RAMS | |||
| CCA | 5'DD(CCA)5 | 95 °C 10 min 95 °C 30 s 64 °C 45 s 72 °C 2 min 72 °C 7 min- 35 cycles | Hantula et al. (1996) |
| GT | 5'YHY(GT)5G | 95 °C 10 min 95 °C 30 s 53 °C 45 s 72 °C 2 min 72 °C 7 min- 35 cycles | Hantula et al. (1996) |
| ACA | 5'BDB(ACA)5 | 95 °C 10 min 95 °C 30 s 49 °C 45 s 72 °C 2 min 72 °C 7 min- 35 cycles | Hantula et al. (1996) |
| CGA | 5'DHB(CGA)5 | 95 °C 10 min 95 °C 30 s 60 °C 45 s 72 °C 2 min 72 °C 7 min- 35 cycles | Hantula et al. (1996) |
| REP-PCR | |||
| BOX1A | 5′-CTACGGCAAGGCGACGCTGACG-3′ | 95 °C 7 min, 94 °C 1 min, 53 °C 1 min, 65 °C 8 min, 65 °C 16 min- 30 cycles | Versalovic et al. (1991) |
| ERIC1R | 5′-ATGTAAGCTCCTGGGGATTCAC-3′ | ||
| ERIC2 | 5′-AAGTAAGTGACTGGGGTGAGCG-3′ | 95 °C 7 min 94 °C 1 min 52 °C 1 min 65 °C 8 min 65 °C 16 min- 30 cycles | Versalovic et al. (1991) |
| REP1R-I | 5′-IIIICGICGICATCIGGC-3′ | ||
| REP2-I | 5′-ICGICTTATCIGGCCTAC-3′ | 94 °C 7 min 94 °C 1 min 40 °C 1 min 65 °C 8 min 65 °C 16 min- 35 cycles | Versalovic et al. (1993) |
| M13 | 5′-GAGGGTGGCGGTTCT-3′ | 93 °C 3 min 93 °C 1 min 55 °C 1 min 72 °C 1 min 72 °C 10 min- 45 cycles | Zaccardelli et al. (2008) |