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. 2023 Dec 2;14:7985. doi: 10.1038/s41467-023-43559-7

Fig. 4. Stopped-flow UV-Vis analysis of Mb-catalyzed cyclopropanation of diazoketone.

Fig. 4

a UV-vis absorption spectra of the reaction of ferrous Mb(H64G,V68A) with BDK (1 mM) observed every 0.05 s for 1 s (red line: 0.05 s; blue line: 1 s) and plot of apparent rate constant (kobs) against BDK concentration; b UV-Vis spectra of the same reaction as in (a) observed from 1 s to four min every 1 s after addition of BDK (red line: 1 s; blue line: 240 s) and plot of apparent rate constant against BDK concentration; c, d UV-Vis spectra of the reaction of ferrous Mb(H64G,V68A) with BDK (1 mM) in the presence of styrene at either c low (3 mM) or d high concentration (20 mM) every 2 s over 4 min. See Supplementary Figs. 3 and 5 for additional data.