TABLE 1.
SacY can antiterminate transcription of a chromosomal bgl-lacZ fusion in E. colia
| Plasmid | Plasmid-encoded protein | Phenotype on MacConkey-lactose mediumb | β-Galatosidase activity (U)c |
|---|---|---|---|
| pMN25 | BglG | + | 47 |
| pSL85-XΔ1 | SacY | + | 39 |
| pOACY | SacY | + | 51 |
| pBR322 | − | 3 |
The experiment was carried out with MA152, an E. coli strain which is Δbgl and carries a bgl-lacZ transcriptional fusion (35).
Expression of the bgl-lacZ fusion was partly determined by colony color on MacConkey-lactose plates: +, red colonies; −, white colonies.
The values, in Miller units, represent the averages of four independent measurements.