Table 1.

Sequence used in this study and melting temperature (T_m) of ASO/RNA or ASO/PNA

	Position		−2	-1	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15
No.	ID	Sequence																			Tm(ºC)a	Target
1	mPCS1	5’	Y	Y	A	C	A	c	c	a	a	g	t	t	c	T	C	c		3’		Pcsk9 (mouse)
2	mPCS1n				A	C	A	c	c	a	a	g	t	t	c	T	C	c
3	mPCS1b			b	A	C	A	c	c	a	a	g	t	t	c	T	C	c
4	mPCS1f				A	C	A	c	c	a	a	g	t	t	c	T	C	c	f
5	PNA(12)-mPCS1	C-term					pT	pG	pG	pT	pT	pC	pA	pA	pG	pA	pG	pG		N-term	79 ± 0.7
6	PNA(11)-mPCS1							pG	pG	pT	pT	pC	pA	pA	pG	pA	pG	pG			74 ± 0.3
7	PNA(10)-mPCS1								pG	pT	pT	pC	pA	pA	pG	pA	pG	pG			63 ± 1.0
8	PNA(C8)-mPCS1				pT	pG	pT	pG	pG	pT	pT	pC									59 ± 2.1
9	cRNA(14)-mPCS1	3’			rU	rG	rU	rG	rG	rU	rU	rC	rA	rA	rG	rA	rG	rG		5’	62 ± 0.1
10	hApo1	5’	Y	Y	A	A	t	g	g	c	c	a	g	c	T	T	G			3’		apoB (human/mouse)
11	hApo1n				A	A	t	g	g	c	c	a	g	c	T	T	G
12	hApo1b			b	A	A	t	g	g	c	c	a	g	c	T	T	G
13	PNA(C5)-hApo1	C-term			pT	pT	pA	pC	pC											N-term	42 ± 0.1
14	PNA(C6)-hApo1				pT	pT	pA	pC	pC	pG											43 ± 0.1
15	PNA(C7)-hApo1				pT	pT	pA	pC	pC	pG	pG										54 ± 0.1
16	PNA(C8)-hApo1				pT	pT	pA	pC	pC	pG	pG	pT									58 ± 0.1
17	PNA(C9)-hApo1				pT	pT	pA	pC	pC	pG	pG	pT	pC								59 ± 0.3
18	PNA(C10)-hApo1				pT	pT	pA	pC	pC	pG	pG	pT	pC	pG							65 ± 0.3
19	PNA(C11)-hApo1				pT	pT	pA	pC	pC	pG	pG	pT	pC	pG	pA						69 ± 0.2
20	PNA(C12)-hApo1				pT	pT	pA	pC	pC	pG	pG	pT	pC	pG	pA	pA					78 ± 0.1
21	PNA(C13)-hApo1				pT	pT	pA	pC	pC	pG	pG	pT	pC	pG	pA	pA	pC				81 ± 0.1
22	PNA(C9)F-hApo1			F	pT	pT	pA	pC	pC	pG	pG	pT	pC
23	cRNA(13)-hApo1	3’			rU	rU	rA	rC	rC	rG	rG	rU	rC	rG	rA	rA	rC			5'	63 ± 0.2

N: LNA, n: DNA, Y: GalNAc_APD, pN: PNA, rN: RNA, b: biotin, f: Alexa647, F: Cy5

^a T_m (ºC) vs. mPCS1n (2 µM), and vs. hApo1n (4 µM).