Table 2.
Sequences with single nucleotide mismatches and melting temperature (Tm). Blue indicates the mismatch nucleotide
| Position | -1 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | |||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Target mRNAs | Sequence | Tm (ºC) | |||||||||||||||
| hApo1nF | 5' | F | A | A | t | g | g | c | c | a | g | c | T | T | G | 3' | |
| PNA(C9)-hApo1 | C-term | pT | pT | pA | pC | pC | pG | pG | pT | pC | N-term | 59 ± 0.3 | |||||
| cGapmer(LNA3) | 3’ | T | t | a | c | c | g | g | t | c | g | a | A | C | 5' | 57 ± 0.2 | |
| cGapmer(LNA4) | T | T | a | c | c | g | g | t | c | g | a | A | C | 60 ± 0.1 | |||
| ApoB | Q | rU | rU | rA | rC | rC | rG | rG | rU | rC | rG | rA | rA | rC | 63 ± 0.2 | ||
| Copg | Q | rU | rU | rA | rC | rC | rG | rG | rG | rC | rG | rA | rA | rC | 54 ± 0.1 | ||
| Mast2 | Q | rU | rU | rA | rC | rC | rG | rG | rU | rG | rG | rA | rA | rC | 50 ± 0.2 | ||
| Hltf | Q | rU | rU | rA | rC | rC | rG | rG | rU | rU | rG | rA | rA | rC | 51 ± 0.2 | ||
N: LNA, n: DNA, pN: PNA, F: TAMRA, Q: BHQ2, rN: RNA.