Table 3.
Sequence used in this study and melting temperature (Tm) of ASO/RNA or ASO/PNA
| Position | −3 | −2 | -1 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| No. | ID | Sequence | Tm (ºC)a | Target | ||||||||||||||||||||||
| 1 | Acsl1 | 5' | Y | Y | Y | C | T | C | c | a | t | g | a | c | a | c | a | g | c | a | T | T | a | 3' | Acsl1 (human/mouse) | |
| 2 | Acsl1n | C | T | C | c | a | t | g | a | c | a | c | a | g | c | a | T | T | a | |||||||
| 3 | Acsl1b | b | C | T | C | c | a | t | g | a | c | a | c | a | g | c | a | T | T | a | ||||||
| 4 | pPNA(C14)-Acsl1 | N-term | pT | pA | pC | pT | pG | pT | pG | pT | pC | pG | pT | pA | pA | pT | C-term | 52 ± 0.3 | ||||||||
| 5 | pPNA(C15)-Acsl1 | pG | pT | pA | pC | pT | pG | pT | pG | pT | pC | pG | pT | pA | pA | pT | 55 ± 0.4 | |||||||||
| 6 | pPNA(C16)-Acsl1 | pG | pG | pT | pA | pC | pT | pG | pT | pG | pT | pC | pG | pT | pA | pA | pT | 61 ± 0.8 | ||||||||
| 7 | pPNA(C17)-Acsl1 | pA | pG | pG | pT | pA | pC | pT | pG | pT | pG | pT | pC | pG | pT | pA | pA | pT | 67 ± 0.9 | |||||||
| 8 | pPNA(C18)-Acsl1 | pG | pA | pG | pG | pT | pA | pC | pT | pG | pT | pG | pT | pC | pG | pT | pA | pA | pT | 73 ± 0.2 | ||||||
| 9 | cRNA(18)-Acsl1 | 3’ | rG | rA | rG | rG | rU | rA | rC | rU | rG | rU | rG | rU | rC | rG | rU | rA | rA | rU | 5’ | 65 ± 0.2 | ||||
N: LNA, n: DNA, Y: GalNAcAPD, pN: PNA, rN: RNA, b: biotin.
aTm vs Acsl1n (4 µM).