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. Author manuscript; available in PMC: 2023 Dec 3.
Published in final edited form as: Biochemistry. 2019 Sep 26;58(40):4112–4124. doi: 10.1021/acs.biochem.9b00593

Figure 2. Deamidation at γS N14 and N76 is associated with insolubility in aged human lenses.

Figure 2.

(A) Photographs of 82 and 85-year old lenses. (B) Brunescence estimated by measuring loss of intensity of blue light as yellowing lenses preferentially absorb blue light. (C and D) The percent deamidation at γS N14 and N76, respectively, were measured by mass spectrometry of tryptic peptides from the nuclear soluble (NS) and nuclear insoluble (NI) fractions of lenses 1 and 2 shown in (A).