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SDS-PAGE analysis of enoyl-CoA hydratase from E. coli BL21(DE3)/pETNB3. Lanes: 1, molecular mass standard proteins, with the masses indicated on the left (from top to bottom, phosphorylase b, bovine serum albumin, ovalbumin, carbonic anhydrase, and soybean trypsin inhibitor); 2, crude extract proteins of E. coli BL21(DE3)/pETNB3; 3, purified enoyl-CoA hydratase after chromatography on Q-Sepharose.
