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Elution profile of enoyl-CoA hydratase from E. coli BL21(DE3)/pETNB3. The soluble protein fraction from the cells grown in four 100-ml cultures was applied to a Q-Sepharose column. ░⃞, absorbance at 280 nm; ——, concentration of NaCl; •, enoyl-CoA hydratase activity toward crotonyl-CoA.
