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Purification of enoyl-CoA hydratase from E. coli BL21(DE3)/pETNB3
| Purification step | Vol (ml) | Protein
|
Total activitya (U) | Sp acta (U/mg) | Yield (%) | Purification (fold) | |
|---|---|---|---|---|---|---|---|
| mg | mg/ml | ||||||
| None (crude enzyme) | 10 | 101 | 10.1 | 1.7 × 105 | 1.7 × 103 | ||
| Q-Sepharoseb | 5 | 21.6 | 4.3 | 1.1 × 105 | 5.1 × 103 | 65 | 3 |
Enoyl-CoA hydratase activity toward crotonyl-CoA (0.25 mM).
Enzyme was concentrated and desalted after elution from a Q-Sepharose column.
