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Hexokinase activity in the mutants
| Strain | Sp act (mU/mg)a
|
||
|---|---|---|---|
| Hexokinase
|
Glucose-6-P dehydrogenase | ||
| Glucose | Fructose | ||
| Sp972 | 88 | 153 | 43 |
| 3/23 | 172 | 190 | 65 |
| 3/8 | 200 | 224 | 96 |
Cultures were grown in YES medium containing 1% glycerol, 0.4% ethanol, and 10 mM δ-gluconolactone to the log phase. Enzymes were assayed in cell extracts, and enzyme activity has been expressed in milliunits per milligram of protein.
