TABLE 4.
Constitutive expression of shunt pathway enzymes in the wild type and mutant
| Enzymea | Sp act (mU/mg)b
|
|||
|---|---|---|---|---|
| Sp972 h−
|
3/23-1T1A
|
|||
| Glucose | Glycerol + ethanol + δ-gluconolactone | Glucose | Glycerol + ethanol + δ-gluconolactone | |
| ZWF | 220 | 152 | 132 | 209 |
| TAL | 6 | 13 | 20 | 13 |
| TKT | 6 | 15 | 15 | 17 |
| PGL | 3 | 12 | 4 | 5 |
| GNK | 27 | 56 | 43 | 46 |
| GND | 552 | 1,549 | 1,196 | 1,255 |
| PPI | 4 | 22 | 7 | 8 |
| PPE | 16 | 50 | 53 | 45 |
a
ZWF, glucose-6-P dehydrogenase; TAL, transaldolase; TKT, transketolase; PGL, phosphogluconolactonase; GNK, gluconate kinase; GND, 6-phosphogluconate dehydrogenase; PPI, pentose phosphate isomerase; PPE, pentose phosphate epimerase.
b
Cultures were grown on complete synthetic medium containing 1% glycerol, 0.4% ethanol, and 10 mM δ-gluconolactone to the log phase. The cells were harvested, washed and inoculated into complete synthetic medium containing 1% glycerol, 0.4% ethanol, and 10 mM δ-gluconolactone. Enzymes were assayed in the cell extracts, and enzyme activity has been expressed in milliunits per milligram of protein.