Table 3.
Means, medians, and 95% CIs for immune cell densities within whole tissue areas (combined intraepithelial and stromal areas) in patients treated with neoadjuvant chemoradiotherapy (CRT) plus pembrolizumab or neoadjuvant CRT alone
| Tumor immune microenvironment (cells/mm2) | ||||||||
| Immune cell subset |
Arm A | Arm B | Arms A–B | Arm A | Arm B | Arms A–B | ||
| Mean (SD) |
Mean (SD) |
Difference mean |
95% CI Difference mean |
Median (IQR) |
Median (IQR) |
Difference median |
95% CI Difference median |
|
| T-cell panel | n=17 | n=7 | n=17 | n=7 | ||||
| CD8+ | 119.8 (140.5) |
83.9 (97.1) |
35.9 | −70.1 to 142.0 | 67.4 (39.3-141.8) |
37.9 (22.9-173.4) |
29.5 | −89.0 to 148.0 |
| CD8+Ki67+ | 17.1 (21.7) |
21.6 (46.1) |
−4.5 | −47.3 to 38.4 | 7.6 (3.2-29.6) |
2.8 (1.4-15.2) |
4.8 | −9.8 to 19.5 |
| CD4+ | 112.5 (80) |
124.3 (126.9) |
−11.8 | −130.9 to 107.4 | 119.3 (46.1-156.3) |
48.4 (40.7-223.8) |
70.8 | −63.9 to 205.6 |
| CD4+FOXP3+ | 11.3 (15.8) |
20.9 (28.4) |
−9.6 | −36.1 to 16.9 | 5.7 (2.1-13.5) |
4.8 (2.1-38.7) |
0.9 | −23.5 to 25.3 |
| CD4+Ki67+ | 6.1 (6.5) |
7.6 (8.2) |
−1.5 | −9.3 to 6.3 | 3.4 (1.9–8.0) |
7.0 (0.7-14.6) |
−3.6 | −13.3 to 6.1 |
| Myeloid-cell subsets | n=15 | n=7 | n=15 | n=7 | ||||
| CD15+ granulocytes | 79.3 (136.8) |
51.0 (63.2) |
28.3 | −60.6 to 117.3 | 14.9 (5.9-90.5) |
17.2 (10.7-110.8) |
−2.4 | −78.3 to 73.5 |
| CD15+ARG1+ | 42.0 (67.0) |
22.1 (26.7) |
19.9 | −21.9 to 61.7 | 4.2 (1.8-71.9) |
9.0 (2.0-38.8) |
−4.8 | −44.6 to 35.0 |
| CD15+ARG1– | 28.5 (55.6) |
16.8 (18.8) |
11.6 | −21.9 to 45.1 | 11.9 (2.1-32.9) |
8.7 (3.7-27.3) |
3.1 | −17.6 to 23.8 |
| CD14+ monocytes | 355.2 (245.7) |
555.9 (530.3) |
−200.7 | −694.6 to 293.2 | 320.6 (129.1-539.1) |
281.1 (189.1-767.1) |
39.6 | −393.1 to 472.2 |
| Macrophages | n=12 | n=7 | n=12 | n=7 | ||||
| Total macrophages | 1139.4 (1417.1) |
1020.8 (879.5) |
118.6 | −994.2 to 1231.5 | 802.3 (482.3-943.4) |
597.9 (511.3-1564.1) |
204.4 | −534.0 to 942.7 |
| M1-polarized macrophages | 365.9 (785.9) |
133.5 (103.7) |
232.4 | −270.9 to 735.8 | 101.3 (52.4-180.0) |
107.6 (44.6-205.9) |
−6.3 | −136.6 to 124.0 |
| M2-polarized macrophages | 213.0 (139.3) |
343.2 (288.8) |
−130.1 | −400.5 to 140.2 | 199.5 (107.4-239.2) |
325.6 (70.1-487.7) |
−126.1 | −407.7 to 155.5 |
CI, Confidence Interval; IQR, Interquartile range; SD, Standard Deviation.