FIG. 2.

SDS-PAGE of the native binding protein as isolated from the membrane of T. litoralis. Lanes: 1, membrane preparation from the uninduced strain (no yeast extract in the medium); 2, membrane preparation from the induced strain (with yeast extract in the medium); 3, purified protein containing the lipid anchor; 4, water-soluble hybrid protein (the construct carries the cleavable signal sequence of E. coli at its N terminus) isolated from the E. coli periplasm (as a reference); St, molecular mass standards (from top to bottom: 66, 45, 36, 29, 24, 20.1, and 14.2 kDa).