FIG. 3.

Fluorescence changes of TMBP in the presence of trehalose and maltose. The recordings were done consecutively. First, the middle trace was obtained after temperature equilibration without addition of substrate. The upper trace was then recorded after the addition of 1 μM (final concentration) maltose, followed by the lower trace after the addition of 100 μM trehalose. Similar tracings in the reverse order were obtained when the order of the additions was reversed, i.e., first 1 μM trehalose and then 100 μM maltose (data not shown). Conditions were as follows: temperature, 55°C; excitation, 280 nm; TMBP concentration, 16 μg/ml; buffer, 50 mM Tris-HCl (pH 7.5) containing 0.6% LDAO.