FIG. 8.

PAGE under nondenaturing conditions and in the presence of radioactively labeled trehalose. Lanes: 1, cytoplasmic extract (not heat treated) of cells containing pRHo1000 (construct A in Fig. 1); 2, purified periplasmic TMBP (construct A in Fig. 1); 3, heterologously expressed wild-type TMBP (construct C in Fig. 1) solubilized in 1% octyl-β-glucoside; 4, purified TMBP solubilized in 1% octyl-β-glucoside. The gel was dried and autoradiographed. Staining was indicative of trehalose binding. Lane 3 contains two forms of the protein, one of which hardly entered the gel.