Fig. 1.

Real-time polymerase chain reaction (RT-PCR) analysis of 3 differentially expressed genes in Acanthamoeba. A. castellanii ingested E. coli (A+E) and L. pneumophila (A+L) for 12 h, and the gene expression ACA1_077100, ACA1_175060, and AFD36229.1 was determined by RT-PCR. (A) ACA1_077100, (B) ACA1_175060, and (C) AFD36229.1. Data are expressed as mean±SD from 3 individual experiments. Asterisks indicate statistical significance compared to the Acanthamoeba control (A), as determined by Student’s t-test (*P<0.05, **P<0.01, ****P<0.0001).