Fig. 2.

Real-time polymerase chain reaction (RT-PCR) analysis of the 3 differentially expressed genes in Acanthamoeba after transfection of siRNA. A. castellanii were transfected with siRNA and coincubated with either E. coli (A+E) or L. pneumophila (A+L) for 12 h. siRNA-mediated gene silencing was confirmed by RT-PCR. (A) ACA1_077100, (B) ACA1_175060, and (C) AFD36229.1. Data are expressed as mean ±SD from 3 individual experiments. Asterisks indicate that the means are significantly different compared to untransfected Acanthamoeba (***P<0.001, ****P<0.0001).