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. 2023 Nov 28;61(4):397–404. doi: 10.3347/PHD.23088

Fig. 3.

Fig. 3

siRNA-transfected Acanthamoeba containing E. coli. Acanthamoeba that did and did not undergo siRNA transfection were cultured with E. coli for 12 h. Those containing E. coli were stained with Giemsa staining solution. (A) A. castellanii control, (B) A. castellanii containing E. coli, (C) siRNA-transfected A. castellanii control, (D) ACA1_077100 siRNA-transfected A. castellanii containing E. coli, (E) ACA1_175060 siRNA-transfected A. castellanii containing E. coli, and (F) AFD36229.1 siRNA-transfected A. castellanii containing E. coli. Black arrows: E. coli in the cytoplasm of Acanthamoeba. Red arrows: E. coli in the phagosomes of Acanthamoeba. Bar=10 μm.