FIGURE 3.

Influence of 39-3x cell culture viability on the resulting levels of RBD (319-541)-His6 dimers produced in the batch culture supernatant. In (A), the concentration of dimeric RBD, as measured by ELISA and shown in the table, corresponds to the level of RBD dimers detected in the culture supernatant by non-reducing Western blot with an anti-RBD antibody (Panel (B), top blot). The reduced Western blot (Panel (B), bottom blot) was revealed with an anti-His tag antibody. In (C), the area of dimeric RBD detected by both Western blot and ELISA is shown relative to cell viability.