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. 2023 Nov 17;11:1287551. doi: 10.3389/fbioe.2023.1287551

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Copyright © 2023 Boggiano-Ayo, Palacios-Oliva, Lozada-Chang, Relova-Hernandez, Gomez-Perez, Oliva, Hernandez, Bueno-Soler, Montes de Oca, Mora, Machado-Santisteban, Perez-Martinez, Perez-Masson, Cabrera Infante, Calzadilla-Rosado, Ramirez, Aymed-Garcia, Ruiz-Ramirez, Romero, Gomez, Espinosa, Gonzalez, Cabrales, Guirola, de la Luz, Pi-Estopiñan, Sanchez-Ramirez, Garcia-Rivera, Valdes-Balbin, Rojas, Leon-Monzon, Ojito-Magaz and Hardy.

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ESI-MS spectra of RBD (319-541)-His6 species produced on a large scale (500 L). The spectra correspond to samples that were deglycosylated with PNGase F in the presence of 5 mM N-ethylmaleimide and digested in solution with trypsin. The top panel displays spectra of RBD monomer from batches 5028/P2109 (A), 5028/P2110 (B), and 5028/P2111 (C). The bottom panel shows spectra of RBD dimer from batches 5016/P2116 (D), 5016/P2117 (E), and 5016/P2118 (F). The spectra insets provide magnified m/z regions, and the O-glycan structures conform to the SNFG (symbol nomenclature for glycans) system. The S-S notation refers to peptides with disulfide bonds.