TABLE 1.
List of C. tropicalis strains used in this study
| Strain | Genotypea | Presence or absence of thiolase genes (T1A/T1B T3A/T3B) |
|---|---|---|
| Representative | ||
| SU-2b | ura3a/ura3b T1A/T1B T3A/T3B | +/+ +/+ |
| K6 | ura3a/ura3b t1aΔ::lacZ/T1B T3A/T3B | −/+ +/+ |
| K8 | ura3a/ura3b T1A/t1bΔ::lacZ T3A/T3B | +/− +/+ |
| K68U | ura3a/ura3b t1aΔ::URA3/t1bΔ::lacZ T3A/T3B | −/− +/+ |
| K7 | ura3a/ura3b T1A/T1B t3aΔ::lacZ/T3B | +/+ −/+ |
| K0 | ura3a/ura3b T1A/T1B T3A/t3bΔ::lacZ | +/+ +/− |
| K70 | ura3a/ura3b T1A/T1B t3aΔ::lacZ/t3bΔ::lacZ | +/+ −/− |
| K870 | ura3a/ura3b T1A/t1bΔ::lacZ t3aΔ::lacZ/t3bΔ::lacZ | +/− −/− |
| K6870U | ura3a/ura3b t1aΔ::URA3/t1bΔ::lacZ t3aΔ::lacZ/t3bΔ::lacZ | −/− −/− |
| K8Δ | ura3a/ura3b T1AΔC6/t1bΔ::lacZ T3A/T3B | ΔC/− +/+ |
| K870Δ | ura3a/ura3b T1AΔC6/t1bΔ::lacZ t3aΔ::lacZ/t3bΔ::lacZ | ΔC/− −/− |
| Intermediate | ||
| K6ZUZ | ura3a/ura3b t1aΔ::ZUZ/T1B T3A/T3B | |
| K8ZUZ | ura3a/ura3b T1A/t1bΔ::ZUZ T3A/T3B | |
| K7ZUZ | ura3a/ura3b T1A/T1B t3aΔ::ZUZ/T3B | |
| K0ZUZ | ura3a/ura3b T1A/T1B T3A/t3bΔ::ZUZ | |
| K70ZUZ | ura3a/ura3b T1A/T1B t3aΔ::lacZ/t3bΔ::ZUZ | |
| K870ZUZ | ura3a/ura3b T1A/t1bΔ::ZUZ t3aΔ::lacZ/t3bΔ::lacZ | |
| K8ΔU | ura3a/ura3b t1aΔ::(T1AΔC6::URA3)/t1bΔ::lacZ T3A/T3Bc | |
| K870ΔU | ura3a/ura3b t1aΔ::(T1AΔC6::URA3)/t1bΔ::lacZ t3aΔ::lacZ/t3bΔ::lacZ |
a
Abbreviations: T1A, CT-T1A; T1B, CT-T1B; T3A, CT-T3A; T3B, CT-T3B; T1AΔC6, CT-T1AΔC6; ZUZ, lacZ-URA3-lacZ.
b
Haas et al. (1990) (9).
c
T1AΔC6 represents the gene encoding the mutant Thiolase I, of which the C-terminal 6 amino acid residues were deleted. t1aΔ::(T1AΔC6::URA3) indicates that the linearized plasmid pUT1AΔ containing CT-T1AΔC6 and URA3 is integrated on the CT-T1A locus.