FIGURE 2.

Both CD40 TRAF6 and TRAF2/3 binding motifs on non-B cells are required for EAE. WT or CD40 mutant mice were immunized with rhMOG, and the severity of EAE was examined over time. (A) Clinical score of the indicated mice immunized with rhMOG. (B) Radiation BM chimeras were established by mixing BM from µMT mice with BM from CD40 mutant mice. (C) EAE was induced in the indicated chimeric mice with rhMOG. EAE scores are combined from three independent experiments (mean ± SEM using 10–18 mice per group). Median of the clinical score during days 11–27 for WT mice was compared with each CD40 mutant using a two-tailed nonparametric Mann–Whitney U test. **p < 0.01, ****p < 0.0001. Comparing clinical scores of CD40^−/− mice with CD40ΔT6 (p < 0.001), CD40ΔT2 (p < 0.01), or CD40ΔT2* (p < 0.01) mice also revealed statistically significant differences.