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SDS-PAGE of His-tag-purified proteins. Lane 1, BchI(2) (38 kDa); lane 2, BchI (42 kDa); lane 3, BchD (67 kDa); lane 4, BchH (145 kDa). Approximately 0.5 μg of the His-tag-purified or urea-dissolved proteins was loaded onto the gel. The two bands of ca. 80 and 85 kDa present in preparations of BchI(2) and -H proteins are considered to be E. coli-derived contaminants.
