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. 2023 Aug 22;73(9-10):693–712. doi: 10.1007/s12031-023-02143-w

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Tau phosphorylation and pathological conformation are prominent in EC/Tau^ΔK pro-aggregant mice. A Neurons in the MEC show accumulation of Tau phosphorylation at Ser262/Ser356, detected with the 12E8 antibody, in the cell bodies (round shapes) at 6 months of age. The EC of EC/Tau^ΔK (PRO) shows substantially more 12E8 + cells compared to EC/Tau^ΔK−PP (ANTI) mice. No 12E8 + cells are found in WT mice. Scale bar: 50 μm. B Left: Western blot analysis of EC lysates from 6-month-old mice shows that the levels of phospho-Tau (12E8) compared to total Tau (K9JA) are significantly higher in EC/Tau^ΔK (PRO) mice (lane 1) than in EC/Tau^ΔK−PP (ANTI; p = 0.0021, lane 2) or WT mice (p < 0.0001, lane 3). Interestingly, the effect of Tau^ΔK expression was observed for both transgenic human (hTau) and endogenous mouse (mTau). Right: Quantification. One-way ANOVA; post hoc: uncorrected Fisher’s LSD test. Data shown as mean ± SEM. C Pathological conformation of Tau in 6-month-old mice, detected with MC1 antibody, was present only in hTau expressing neurons of EC/Tau^Δ.^K (PRO) mice, in the EC and along the perforant path up to the axon terminals where it accumulated in the OML of the DG. Scale bar: 200 μm (upper images); 50 μm (lower images)