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. 2023 Dec 4;14(12):791. doi: 10.1038/s41419-023-06274-1

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Heatmap showing differentially expressed genes (DEGs, FDR < 0.05) in SOX2- overexpressing HCT116 cells based on lncRNA microarray analysis. b qRT-PCR examination of the top ten of most significant DEGs in panel A (mean ± SD; n = 3, two-tailed Student’s t test). c FISH assay detecting the expression and localization of AC005392.2 in tissues of HCT116 and SW620-based xenografts (Scale, 200 μm; inset: scale, 50 μm). d SOX2-overexpressing HCT116 cells or SOX2-deficient SW620 cells were transfected for 72 h with AC005392.2 siRNA or AC005392.2 clone plasmid. Western blotting was performed using the indicated antibodies. e The ECAR was examined in transfected HCT116 cells and SW620 cells using a Seahorse XF assay (mean ± SD; n = 3). f, g Glucose consumption (f) and lactate production (g) were assessed using fluorescence-based kits in transfected HCT116 cells and SW620 cells (mean ± SD; n = 3, two-tailed Student’s t test). h, i Transwell migration assay (h) and tube formation assay (i) were performed in transfected HCT116 cells and SW620 cells (Scale, 200 μm; mean ± SD; n = 3, two-tailed Student’s t test). *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.