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. 2023 Dec 4;14(12):791. doi: 10.1038/s41419-023-06274-1

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a qRT-PCR detection of AC005392.2 expression in the cytoplasmic and nuclear fractions of HCT116 and SW620 cells (mean ± SD; n = 3). b Silver staining showing the stripes pulled down by the sense and antisense AC005392.2 sequences. The arrow indicates the candidate stripes. c Unique peptides of GLUT1 were detected by mass spectrometry. d RIP assay showing the enrichment of AC005392.2 in the complex coprecipitated by anti-GLUT1 antibody in HCT116 and SW620 cells (mean ± SD; n = 3, two-tailed Student’s t test). e, f HCT116 and SW620 cells were transfected for 72 h with an AC005392.2 clone or AC005392.2 shRNA, then treated with 20 μg/ml cycloheximide (e) or 20 μM MG132 (f) for the indicated time. Western blotting was performed to analyze the expression of GLUT1. g, h Immunoprecipitation followed by western blotting was performed to analyze the ubiquitination (g) and SUMOylation (h) of GLUT1 in AC005392.2-overexpressing HCT116 and AC005392.2-knockdown SW620 cells. i Western blotting analysis of the protein levels of GLUT1 in HCT116 cells co-transfected with AC005392.2 clone and SUMO1 siRNA or in SW620 cells co-transfected with AC005392.2 shRNA and SUMO1 clone for 72 h. ***p < 0.001.