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. 2023 Dec 4;14(12):791. doi: 10.1038/s41419-023-06274-1

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a, b Glucose consumption (a) and lactate production (b) were assessed using fluorescence-based kits in HCT116 cells co-transfected with SOX2 clone and GLUT1 siRNA or in SW620 cells co-transfected with SOX2 shRNA and GLUT1 clone for 72 h (mean ± SD; n = 3, two-tailed Student’s t test). c ECAR was examined in transfected HCT116 cells and SW620 cells using a Seahorse XF assay. d Western blotting was performed using the indicated antibodies in transfected HCT116 cells and SW620 cells. e, f Transwell migration assays (e) and tube formation assays (f) were conducted in transfected HCT116 cells and SW620 cells (Scale, 200 μm; mean ± SD; n = 3, two-tailed Student’s t test). g IHC staining of GLUT1 in SOX2-overexpressing HCT116 and SOX2-knockdown SW620 xenografts. Scale, 100 μm. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.