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. 2023 Nov 20;14:1273182. doi: 10.3389/fimmu.2023.1273182

Figure 3.

Figure 3

Keratinocyte-specific STAT3 deficiency predominantly increased the infiltration of type-2 inflammatory cells and the secretion of related cytokines. (A) Analysis of the serum IgE levels (ng/ml) in Stat3f/f mice (n = 8) and Stat3 cKO mice (n = 5) after DNCB treatment using ELISA. (B) Flow cytometry analysis of FcϵRIα+ cells in the dermis of Stat3f/f mice and Stat3 cKO mice (n = 4). (C) Toluidine blue staining of mast cells (red arrows) and H&E staining of eosinophils (black arrows) in mouse ear skin of each group. Bar = 50 μm. (D) Representative IF staining of CD4+ or CD8+ cells (red) in the ear skin of Stat3f/f mice and Stat3 cKO mice. Bar = 50 μm. (E) Statistical analysis results of the number of mast cells, eosinophils, and CD4+ or CD8+ cells (n = 3-4). (F) The expression levels of Il-4, IL-10, TNF-α, and IFN-γ (pg/ml) in the ear skin of Stat3f/f mice and Stat3 cKO mice (n = 3-4). The data are presented as the mean ± SD; P > 0.05 denotes not significant (ns); *P < 0.05, **P < 0.01, and ***P < 0.001.