Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Nov 20;14:1293090. doi: 10.3389/fimmu.2023.1293090

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2023 Couturaud, Doix, Carretero-Iglesia, Allard, Pradervand, Hebeisen and Rufer

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Monomeric TCR-pMHC dissociation rates of CMV/pp65-specific TCRαβ clonotypes in longitudinal analyses. (A) TCR-pMHC dissociation rates (k_off) by wild-type NTAmers of each color-coded CMV-specific TCRαβ clonotypes from donor BCL6 at the indicated time-points. (B) Correlations of NTAmer-based TCR off-rates (k_off) between T_n and T_n+15y obtained from identical TCRαβ clonotypes for CMV/pp65 (left panel) or EBV/BMFL1 (right panel) specificity. Coefficient R² and p-values from simple linear regression analyses are indicated. (C, D) TCR-pMHC off-rates (k_off) by wild-type NTAmers (C; NTA) or mutated NTAmers (D; CD8null NTA) on a representative selection of T cell clones of each identified TCRαβ clonotype and donor. CD8null NTA non-binder clones are represented in the grey boxes and are defined as CD8 binding-dependent clonotypes. The middle line represents the mean. (E) Relative prevalence of each CMV/pp65-specific TCRαβ clonotype at T_n and T_n+15y categorized as high TCR binding avidity/CD8 binding-independent (left panel) or as low TCR binding avidity/CD8 binding-dependent (right panel). Clonotypes are color-coded as in Figure 1D . (F) Ratio of low (CD8 binding-dependent)/high (CD8 binding-independent) TCR binding avidities within the overall CMV/pp65-specific CD8 T cell repertoires at T_n and T_n+15y for each indicated donor. Values >1 (red arrow) indicate an enrichment of low over high avidity CMV/pp65-specific T cell clonotypes. Values <1 (blue arrow) indicate an enrichment of high over low avidity T cell clonotypes. (E, F) p-values by Wilcoxon non-parametric paired test (p > 0.05, not significant).