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. 2023 Nov 20;14:1277582. doi: 10.3389/fimmu.2023.1277582

Figure 1.

Figure 1

Apical expression of SPLUNC1 is decreased in CC16-/- MTECs. (A) Schematic of the experimental design for the mass spectrometry and quantitative proteomics approach to identify apically secreted proteins by WT and CC16-/- MTECs. (B) Volcano plot illustrating the apically secreted proteins identified. Above the horizontal black line represents the cut-off for a p value of <0.05, while the two vertical lines represent the cut-off values of 2-fold change in either the positive or negative direction. Selected antimicrobial proteins are highlighted in red and labeled. (C) Spectral counts of SPLUNC1 protein secreted by WT and CC16-/- MTECs by LC-MS/MS. ***P<0.001 Unpaired t test. Data are presented as mean ± SEM from six independent experiments. (D) SPLUNC1 apical protein expression was confirmed for control-treated WT and CC16-/- MTECs by western blotting. The same volume of protein (20μl) was loaded for each sample. **P<0.01 Unpaired t test. Data are presented as mean±SEM. (E) WT (n=8) and CC16-/- (n=7) MTECs were treated with media (control) for 48 hours, after which BPIFA1 expression was measured by RT-PCR with GAPDH as a housekeeping control. ****P<0.0001 Unpaired t test. Data are presented as mean±SEM.