Figure 5.

Inhibition of VLA-2 results in decreased SPLUNC1 expression and increased Mp burden. (A) BPIFA1 gene expression was measured by RT-PCR in WT MTECs treated with media only (n=3), Mp (n=3), Mp+rCC16 (n=4), Mp+rCC16+BTT3033 (n=4), or Mp+BTT3033 (n=4). GAPDH was used as a housekeeping control. ^*P<0.05, ^**P<0.01 by One-Way ANOVA Tukey’s multiple comparison test. (B) Apical SPLUNC1 protein expression was measured in the same samples from panel A, as well as additional experimental samples to confirm reproducibility, by western blotting. A representative blot is shown. ^*P<0.05, ^**P<0.01 by One-Way ANOVA Tukey’s multiple comparison test. (C) Mp burden was measured in the same samples from panels A by RT-PCR. GAPDH was used as a housekeeping control. ^*P<0.05, ^**P<0.01 by One-Way ANOVA Tukey’s multiple comparison test. Data are presented as mean±SEM.