Table 2.

Quantification of oxylipins. Calibration range, linear coefficient of determination (R2), limit of detection (LOD), lower limit of quantification (LLOQ), mean concentration in HM sEVs.

Oxylipin	Calibrated range (nM)	R2	LOD (nM)	*LLOQ (pM)	HM sEVs Mean ± SD (pM)
17-HDHA	0.29 - 300	0.996	0.09	0.9	21.02 ± 19.64
14-HDHA	0.15 - 300	0.996	0.04	0.5	18.65 ± 17.51
19,20-DiHDPA	0.07 - 300	0.995	0.02	0.2	11.32 ± 8.50
9,10-DiHOME	0.29 - 300	0.998	0.09	0.9	10.61 ± 4.16
12,13-DiHOME	0.15 - 300	0.995	0.04	0.5	7.46 ± 3.09
17,18-DiHETE	0.15 - 300	0.994	0.04	0.5	1.8 ± 1.1
14,15-DiHETRE	0.07 - 300	0.994	0.02	0.2	0.8 ± 0.3
PGF2α	0.07 - 300	0.995	0.02	0.2	0.7 ± 0.3

*referred to HM sEV encountered in the HM sample.