TABLE 2.
Custom primers used in amplification and sequencing experiments
| Primer (Fig. 1 designation) | Sequence (5′→3′) | Description |
|---|---|---|
| 346for-80 (A) | TCTGTCACTTGCTTCTCCTCTTT | Alkyl hydrogen peroxide reductase primer |
| IS1216-698Cy5 (B) | TCTCTTCGGGTTTTCGGTCTG | Nested primer for sequencing from within IS1216 |
| IS1216For (C) | AATTTATTGCGTCTCTTTACTGGA | PCR primer for amplifying IS1216-containing fragments |
| IS1216-110Cy5 (D) | CCCACGGCTACAATAATCACA | Nested primer for sequencing from within IS1216 |
| IS1216Rev (E) | CCGTGGGCTACTATCTTCGTT | PCR primer for amplifying IS1216-containing fragments |
| CH3AT1216 (F) | TGAAGAAACACAAAGGGAATG | Primer downstream of aadE gene; used to read outward from left end of Tn5385 |
| Strep DOWN (G) | GTAAAACCGGCTGCCTGGATAG | Downstream of aadE, toward left end of Tn5385 |
| Strep UP (H) | TGAACCACCCTAAAACAATACCTT | Upstream of aadE ORF, toward Tn5381 |
| JointR (I) | CGTGAAGTATCTTCCTACAGT | Primer reading outward from end of Tn5381 |
| JointL (J) | GGATAAATCGTCGTATCAAAG | Primer reading outward from end of Tn5381 |
| 86-301 (K) | AAATAAAAGAACCGAAATACAGG | Primer downstream of β-lactamase gene; used to read outward from right end of Tn5385 |
| 351 forward (L) | GGACAATTTTAGACCCTGCTGAG | Alkyl hydrogen peroxide reductase primer |