TABLE 1.
Bacterial strains used in this study
| Strain | Relevant genotype | Source or reference |
|---|---|---|
| S. typhimurium | ||
| ATCC 14028-1s | wt1a | American Type Culture Collection |
| SR-11 | wt1a | R. Curtiss III |
| LB5010 | metA22 metE551 ilv-452 leu-3121 trpD2 xyl-404 galE856 hsdLT6 hsdSA29 hsdSB121 rpsL120 | 12 |
| 146 | polA-2 zig214::Tn10 (Tetr) | M. Rhen |
| SF1005 | rpoS::pRR10(ΔtrfA)(Ampr) | D. Guiney |
| UMR1 | ATCC 14028-1s Nalr | This study |
| UMR3 | SR-11 Nalr | This study |
| MAE1 | UMR1 ΔcsgA101::Kmr | This study |
| MAE2 | UMR3 ΔcsgA101::Kmr | This study |
| MAE5 | UMR1 ΔcsgA101 | This study |
| INK1 | SR-11 zcg-101::Kmr | This study |
| MAE40 | SF1005 × UMR1; UMR1 rpoS::pRR10(ΔtrfA)(Ampr) | This study |
| MAE29 | SF1005 × SR-11; SR-11 rpoS::pRR10 (ΔtrfA)(Ampr) | This study |
| MAE46 | UMR1 ΔompR101::Ampr | This study |
| MAE34 | UMR3 ΔompR101::Ampr | This study |
| E. coli K-12 | ||
| DH5α | endA1 hsdR17 supE44 thi-1 recA1 gyrA relA1 Δ(lacZYA-argF)U169 (φ80lacZΔM15) | Laboratory collection |
| MC4100 | F−araD139 Δ(argF-lac)U169 rpsL150 relA1 flbB5301 deoC1 ptsF25 rbsR | Laboratory collection |
| XL1 Blue MR | Δ(mcrA)183 Δ(mcrCB-hsdSMR-mrr)173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac | Stratagene |
| HLO7 | MC4100 Δ(csgG-csgC)::Kmr | 47a |
| MHR204 | MC4100 csgA2::Tn105 Cmr | 34 |
| MHR210 | MC4100 csgG1::Tn105 Cmr | 34 |
| MHR261 | MC4100 csgB2 | 35 |
| MHR426 | MC4100 csgF4 | 36 |
| MHR480 | MC4100 ΔcsgE3 | 37 |
| MHR503 | MC4100 csgD6 | This study |
a
wt1, wild type 1.