TABLE 2.
Plasmids used and constructed in this study
| Plasmid | Characterization | Reference |
|---|---|---|
| pLAFR3 | Tetr RK2 oriT | 64 |
| pMAK700 | Cmr temperature-sensitive replicon derived from pSU101 | 33 |
| pMAK705 | pMAK700 containing pUC19 polylinker | 33 |
| pRK2013 | Kmr ColE1 mob tra | 27 |
| pUC4K | Ampr Kmr cassette | Pharmacia |
| pWSK29 | Ampr pSC101 ori | 67 |
| pWKS30 | Ampr pSC101 ori | 67 |
| pUMR2b | pMAK700 ΔcsgA101; Cmr | This study |
| pUMR2c-1 | pMAK700 ΔcsgA101::Kmr; Cmr Kmr | This study |
| pUMR10-7 | pBS, NsiI-BamHI-cut CSGD1-CSGB3 from SR-11; Ampr | 56a |
| pMU1 | pUC18, PstI fragment of zcg-101; Ampr | This study |
| pMU2 | pUC18, HindIII-PstI fragment of zcg-101; Ampr | This study |
| pMU3a | pMAK705, HindIII-PstI fragment of zcg-101; Cmr | This study |
| pMU3b-1 | pMAK705, zcg-101::Km; Cmr Kmr | This study |
| pMU4 | pLAFR3 containing a 39-kb PstI fragment harboring the csg genes; Tetr Kmr | This study |
| pCSGA | pWKS30, PstI-BamHI-cut CSGB2-CSGC; Ampr | This study |
| pCSGB | pWKS30, HindIII-PstI-cut CSGBD-CSGA1; Ampr | This study |
| pCSGD | pWSK29 (SmaI/EcoRI), EcoRI-cut SP2-SP5; Ampr | This study |
| pCSGE | pWSK29 (SmaI/BamHI), BstYI-cut SP5-SP9; Ampr | This study |
| pCSGF | pWKS30 (EcoRI/SmaI), EcoRI-cut SP6-SP17; Ampr | This study |
| pCSGEFG | pWSK29 (PstI/EcoRI), NsiI-cut CSGD2-SP21; Ampr | This study |
| pUMR7b-2 | pMAK705 ΔompR101::Ampr; Cmr Ampr | This study |