Table 1.
Properties of the samples used for this study and results of DNA extraction with the improved protocol outlined in the Supplementary information.
| Sample | C 1H2 | C 6H6 | C 11H1 | D 2H3 |
|---|---|---|---|---|
| Hole | C | C | C | D |
| Depth (mbsf) | 1.5 | 50.6 | 90.6 | 132.2 |
| Methane (mM) | 0.00 | 0.00 | 4.70 | NA |
| Sulfate (mM) | 27.00 | 16.23 | 0.95 | 2.02 |
| DOC (mM) | 2.84 | 10.88 | 11.19 | 0.63 |
| Remarks | Topmost sample | Upper SMTZ | Anaerobic layer | Basement influenced |
| DNA (ng/μl) | 3.820 | 0.080 | 0.100 | 0.453 |
| Total extracted DNA (ng) | 95.5 | 1.6 | 2.0 | 11.3 |
| extracted DNA/sediment (ng/g) | 22.74 | 0.32 | 0.40 | 2.11 |
The presented information on depth and pore water composition was published at the core data repository of the IODP in 2008 (Ocean Drilling Data, 2021). The DNA concentration of the eluate was determined using the Qubit™ 4 Fluorometer from Invitrogen™. mbsf, meters below seafloor; DOC, dissolved organic carbon; NA, data not available.